M13KE Phage

This product has been discontinued and replaced by E8101.
  • This product was discontinued on 01/01/2017
Catalog #SizeConcentrationPriceQtyAdd to Cart
  
Categories:
Discontinued Products

Description

M13KE Phage is a suspension of infectious virions derived from the Ph.D. cloning vector, M13KE (1). The vector phage may be a useful control for phage ELISA or for titering of phage stocks. For cloning, the isolated replicative form of M13KE is available as part of the Ph.D. Peptide Display Cloning System (NEB #E8101).

Properties and Usage

Affinity Tag

Other

Storage Temperature

-20°C

Sequence Files

Fasta  GenBank  

References

  1. Noren, K. A. and Noren, C. J. (2001). Methods. 23, 169-178.
  2. Sambrook, J. and Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. 3rd ed., 3.17-3.32.

FAQs

  1. What is Ph.D.™ Phage Display?
  2. What peptide libraries are available for use with Ph.D.™ Phage Display?
  3. What strain should i use to titer or amplify M13KE?

Protocols

  1. M13 Amplification
  2. M13 Titer Protocol

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Application Notes

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Absolute Phage Titer:
    Infection of a mid-log culture of E. coli with phage followed by plating, yields ≥ the specified pfu/ml.
  • Cloning Site Verification:
    From infected E. coli, dsDNA is purified. The DNA was sequenced across the MCS and digested with KpnI and EagI to confirm the presence of restriction enzyme cloning sites.
  • Wild-type M13 contamination:
    The product is titered on plates containing X-gal and IPTG to ensure there is no contamination of wild-type M13 phage. Blue plaques are observed at least 500:1 over clear plaques.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.