TransPass™ P Protein Transfection Reagent

  • Catalog # M2563 was discontinued on January 01, 2013
  • Product Information
    TransPass™ P is a protein transfection reagent that can deliver intact functional proteins and peptides with high efficiency into live mammalian cells via endocytosis. The activity of delivered protein is maintained so active enzymes and other bio-molecules such as antibodies can be directly delivered to cells without the need to obtain and transfect an expression construct.

    TransPass P Transfection Reagent forms a non-covalent association with the protein protecting it from degradation upon endocytosis, and thus, allowing the study of protein activity in living cells without the need of permeabilization and fixation.

    This protein tranfection reagent has a broad range of cell-type specificity, minimal toxicity, and a high transfection efficiency. A fluorescently labeled control protein (lgG labeled with a 549 fluorophore) is included for optimization of transfection conditions.

    Cell Lines Successfully Transfected:
    CHO-K1, COS-7, HeLa, HepG2, Huh-7, HUVEC, IMR-90, MCF-7, MEF, and NIH3T3.

    Figure 1:
    NIH3T3 cells transfected for 2 hours with 6 µl TransPass P Protein Transfection Reagent and 1 µg IgG labeled with a fluorescent dye (a 549 fluorophore). The cells were fixed, DAPI stained and photographed 24 hours post-transfection.
    Figure 2:
    HUVEC cells transfected with an antibody labeled with Oregon Green and TransPass P Transfection Reagent. 1µg of antibody was delivered with 2 µl of TransPass P. The cells were fixed and photographed 24 hours post- transfection.
    Figure 3:
    NIH-3T3 cells were transfected with 1 µg of a fluorescent peptide (Flu-P1 NEB#P6606) and the indicated amounts of TransPass P Transfection Reagent in a 6-well plate format. The cells were photographed 24 hours post-transfection.
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