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  • TransPass™ P Protein Transfection Reagent

    Discontinued Date

    01/01/2013
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    Categories:
    Discontinued Products

    Description

    TransPass™ P is a protein transfection reagent that can deliver intact functional proteins and peptides with high efficiency into live mammalian cells via endocytosis. The activity of delivered protein is maintained so active enzymes and other bio-molecules such as antibodies can be directly delivered to cells without the need to obtain and transfect an expression construct.

    TransPass P Transfection Reagent forms a non-covalent association with the protein protecting it from degradation upon endocytosis, and thus, allowing the study of protein activity in living cells without the need of permeabilization and fixation.

    This protein tranfection reagent has a broad range of cell-type specificity, minimal toxicity, and a high transfection efficiency. A fluorescently labeled control protein (lgG labeled with a 549 fluorophore) is included for optimization of transfection conditions.

    Cell Lines Successfully Transfected:
    CHO-K1, COS-7, HeLa, HepG2, Huh-7, HUVEC, IMR-90, MCF-7, MEF, and NIH3T3.

    Figure 1:
    NIH3T3 cells transfected for 2 hours with 6 µl TransPass P Protein Transfection Reagent and 1 µg IgG labeled with a fluorescent dye (a 549 fluorophore). The cells were fixed, DAPI stained and photographed 24 hours post-transfection.
    Figure 2:
    HUVEC cells transfected with an antibody labeled with Oregon Green and TransPass P Transfection Reagent. 1µg of antibody was delivered with 2 µl of TransPass P. The cells were fixed and photographed 24 hours post- transfection.
    Figure 3:
    NIH-3T3 cells were transfected with 1 µg of a fluorescent peptide (Flu-P1 NEB#P6606) and the indicated amounts of TransPass P Transfection Reagent in a 6-well plate format. The cells were photographed 24 hours post-transfection.

    Properties and Usage

    Storage Temperature

    4°C

    Notes

    1. Use purified proteins and enzymes free of bacteria or toxins.
    2. The amount of protein and the incubation time with the transfection complex mixture used may be adjusted for optimal transfection efficiency.
    3. Use 0.5-1 µl of the control protein per transfection (6-well plate format).
    4. Storage: TransPass P Transfection Reagent should be stored at 4°C. The fluorescent-labeled control protein (lgG labeled with a 549 fluorophore) should be protected from light and stored at 4°C.
    5. TransPass P is a proprietary formulation manufactured by Targeting Systems.

    FAQs

    1. Cellular Imaging and Analysis FAQs

    Protocols

    1. TransPass P Protein: Transfection Protocol

    Selection Tools

    Quality Control

    Quality Assurance Statement

    • Each lot of transfection reagent is tested for efficient delivery of the control protein.

    Material Safety Data Sheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.