- This product was discontinued on January 01, 2013
TransPass™ V is an Adenovirus-derived enhancer component that is used along with a transfection reagent. The combination of a TransPass transfection reagent & TransPass-V significantly enhances transfection efficiency in many cell lines and primary cells including endothelial or epithelial (1,2). For example, the combination of TransPass D2 & TransPass V yields optimal plasmid transfection efficiency in suspension cells (Figure 1). This combination has low toxicity and thus, transfected cells show very little, if any, cell death (Figure 2).
*TransPass-V contains a replication-deficient Adenovirus preparation. Because of the nature of this component, it should not be used with cell lines that contain Adenovirus sequences such as HEK-293, to avoid complementation of the virus. Additionally, it is recommended that common laboratory biosafety used in standard Adenovirus work is practiced. For more information see http://oba.od.nih.gov/oba/index.html.
TransPass V is a proprietary formulation manufactured by Targeting Systems. Please direct all inquirires regarding reagent composition to Targeting Systems.
Cell Lines Successfully Transfected:
- MEF (TransPass D2 & TransPass V)
- IMR-90 (TransPass D2 & TransPass V)
- HepG2 (TransPass D2 & TransPass V)
- Huh-7 (TransPass D1, TransPass D2 & TransPass V)
- MDCK (TransPass D1, TransPass D2 & TransPass V)
- A549 (TransPass R1 & TransPass V)
- T47D (TransPass R1 & TransPass V)
- Jurkat (TransPass D2, TransPass R1 & TransPass V) (Figure 1)
- Use early passages of cells (up to the 6th passage) for transfections.
- Use non-collagen and non-gelatin coated tissue culture plates only (transfection of cells plated on collagen-coated surfaces may result in lower transfection efficiency).
- Recommended media: MCDB131 from VEC technologies, Media 199 plus supplements and 20% fetal calf serum or EBM from Cambrex plus supplements and 10% fetal calf serum. Cells grown in these media appear healthier and give higher transfection efficiency.
- Clonetics HUVEC cells have given very good transfection results.
- For consistent results, it is important to maintain healthy proliferating cells that are regularly passaged.
- If cells have been grown in medium containing heparin, they must be washed after trypsinizing and resuspended in growth medium without heparin and antibiotics/antimycotics before plating for transfection.
- It is important that NO heparin and NO antibiotics/antimycotics in the growth medium during transfection.
- Use sterile plasmid DNA purified by CsCl gradient centrifugation or column chromatography.
- The amount of plasmid DNA per transfection can be varied, and the ratio of HUVEC Reagent Component to TransPass-V should be kept between (1:1) and (1:2).
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