TransPass™ D2 Transfection Reagent

  • This product was discontinued on 01/01/2013
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M2554
  • Discontinued
    Discontinued
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    The TransPass D2 Transfection Reagent is a non-lipid cationic polymer designed for transfecting plasmid DNA into many mammalian cell lines including primary cell lines and endothelial cell lines. Complex co-transfections of many plasmids can be efficiently transfected using the TransPass D2 Transfection Reagent.


    Cell Lines Successfully Transfected:
    A549, CHO-K1, COS, HCT116, HEK293, HeLa, HepG2, HL60, Jurkat, MCF-7, MDA, NIH3T3 and U20S.

    Figure 1:
    Human Umbilical Vein Endothelial Cells (HUVEC) transfected with a plasmid expressing GFP using TransPass D2 Transfection Reagent (Pradhan et al., unpublished results).
    Figure 2:
    Titration of TransPass D2 Transfection Reagent in CHO-K1 cells using Protocol 1 and secreted Gaussia luciferase (pCMV-GLuc) as a reporter. Transfections contain 0.7 µg pCMV-GLuc and 250 µl plating medium (10%FBS-F12K) per well. The values obtained from the transfections with the indicated amounts of TransPass D2 Transfection Reagent were obtained from 20 µL of culture medium 24 hr post transfection.
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