TransPass™ D1 Transfection Reagent

  • Catalog # M2553 was discontinued on January 01, 2013
  • Product Information

    The TransPass D1 Transfection Reagent is a formulation of cationic lipid-vesicles designed for transfecting plasmid DNA into many mammalian cell lines including primary endothelial cells. It is a frozen suspension that should be resuspended by vortexing before use. Complex co-transfections of many plasmids can be efficiently transfected using the TransPass D1 Transfection Reagent.

    Cell Lines Successfully Transfected:
    A549, COS, Drosophilia SL2, HEK293, HeLa, HO23, NIH3T3 and U20S.

    Primary Endothelial cells: HUVEC, HMVEC, lung microvascular endothelial cells, human aortic endothelial cells, bovine aortic endothelial cells.

    Figure 1:
    Primary human hepatocytes transfected with a plasmid expressing β-galactosidase using TransPass D1 Transfection Reagent.
    Figure 2:
    Cells transfected in 12 well plate format with 1.3 µg pCMVGLuc Control Plasmid and 3.9 µl TransPass D1 Transfection Reagent. Assays were performed in 20 µl cell supernatant 24 hr post-transfection.

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