TransPass™ R2 Transfection Reagent
- This product was discontinued on January 01, 2013
* This reagent has two solutions: Solution A - 0.5 ml, Solution B - 1.0 ml
The TransPass™ R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection of siRNA in a variety of mammalian cell lines that include “difficult to transfect" cells such as primary cells, endothelial cells, lymphocytes, muscle cells, etc. It shows reduced levels of cell toxicity as compared to cationic liposome-based reagents.
NEB has determined that in addition to synthetic siRNAs, siRNA mixtures made with ShortCut® RNase III (NEB #M0245) can be very efficiently transfected in many mammalian cell lines with the TransPass™ R2.
Efficient delivery of labeled siRNA and silencing of endogenous genes has been achieved in many cell lines including:
A549, COS-7, HEK293, HepG2, NIH3T3, HeLa, OVCAR-3, HUVEC, HMVEC, THP-1, B-lymphoma cells, vascular smooth muscle cells, human lens epithelial cells.
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