Tth RecA

Developed and produced by BioHelix Corp., a NEB-affiliated company.
  • This product was discontinued on 01/27/2016
heat inactivation no
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Description

Tth RecA is a RecA homolog isolated from Thermus thermophilus. It has a ssDNA-dependent ATPase activity at an optimal temperature between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing.

Highlights

  • Extreme thermostability
  • Nucleic acid amplification and sequencing
  • Isolated from a recombinant source

Product Source

An E. coli strain that carries the cloned RecA gene from Thermus thermophilus

Properties and Usage

Unit Definition

Sold by mass of pure protein as determined by OD280.

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.5 @ 25°C

Heat Inactivation

No

Notes

  1. Tth RecA is active in any polymerase buffer. Add 400 ng of the Tth RecA per 50 µl reaction.

References

  1. Wasserman, S.A. and Cozzarelli, N.R. (1985). Proc. Natl. Acad. Sci. USA. 82, 1079-1083.
  2. Biet, E. et al. (2001). Biochemistry. 40, 1779-1786.
  3. Shortle, D. et al. Proc. Natl. Acad. Sci. USA. 77, 5375-5379.
  4. Rigas, B. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9591-9595.
  5. Honigberg, S.M. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9586-9590.
  6. Koob, M. et al. (1992). Nucleic Acids Res.. 20, 5831-5836.
  7. Shigemori, Y. et al. (2005). Nucleic Acids Res.. e126.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Quality Control

Quality Assurance Statement

  • Each lot is tested for its ability to form a stable triple helix and is visually determined to be > 99% pure on an SDSpolyacrylamide gel.

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Exonuclease Activity (Radioactivity Release):
    The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.