Cas9 Nuclease NLS, S. pyogenes

This product has been discontinued on 10/1/16, and replaced by EnGen™ Cas9 NLS, S. pyogenes (NEB #M0646).

  • This product was discontinued on October 01, 2016
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Ordering Information

M0641
  • Discontinued
    Discontinued
  • Product Information
    sgRNA Designer Tool














    Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein.

    Cas9 nuclease, S. pyogenes, complexed with an sgRNA
    Cas9
    Cleavage occurs three nucleotides upstream of the PAM sequence (shown in red). sgRNAs are complimentary to the strand of DNA opposite of the PAM.

    Product Source

    An E. coli strain that carries the cloned Cas9 gene from Streptococcus pyogenes with a C-terminal Simian virus 40 (SV40) nuclear localization signal (NLS) and a N-terminal 6XHis tag.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    Cas9 Nuclease Reaction Buffer -20 10 X
    Product Categories:
    Nucleases,
    Discontinued
    Applications:
    Cloning & Synthetic Biology
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