OneTaq® Quick-Load® 2X Master Mix with GC Buffer
- This product was discontinued on January 02, 2018
How to Amplify GC-rich DNA M0491 M0480
5 Tips for Setting Up Your PCR
Why is Tm Important in Primer Design?
Overview of PCR
OneTaq® Quick-Load® 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® High GC Enhancer -20 50 %
- Product Categories:
- Discontinued Products
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.