OneTaq® Quick-Load® 2X Master Mix with GC Buffer
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Overview of PCR
OneTaq® Quick-Load® 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® High GC Enhancer -20 50 %
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