OneTaq® 2X Master Mix with GC Buffer
- This product was discontinued on January 02, 2018
The ONE polymerase for your endpoint PCR needs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA Polymerases for use with routine and difficult PCR experiments.
- Obtain high yields with GC rich templates (>65%)
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
How to Amplify GC-rich DNA M0491 M0480
5 Tips for Setting Up Your PCR
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Overview of PCR
OneTaq® 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR™ DNA Polymerases ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient Master Mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® High GC Enhancer -20 50 %
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