ATP Sulfurylase

Description

ATP Sulfurylase catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.

Highlights

  • Isolated from a recombinant source

Product Source

An E.coli strain carrying a plasmid expressing the S. cerevisiae gene MET3.

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of APS and PPi to ATP in one minute at 30°C in a total reaction volume of 40 µl.

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
50 mM NaCl
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5

Unit Assay Conditions

115 mM Tris-HCl (pH 8.0), 0.58 mM β-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5´-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
  • Phosphatase Activity (PNPP):
    The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation the percent degradation is determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.