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  • ATP Sulfurylase

    Description

    ATP Sulfurylase catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.

    Highlights

    • Isolated from a recombinant source

    Product Source

    An E.coli strain carrying a plasmid expressing the S. cerevisiae gene MET3.

    Properties and Usage

    Unit Definition

    One unit is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of APS and PPi to ATP in one minute at 30°C in a total reaction volume of 40 µl.

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    50 mM NaCl
    0.1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 7.5

    Unit Assay Conditions

    115 mM Tris-HCl (pH 8.0), 0.58 mM β-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5´-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • Phosphatase Activity (PNPP):
      The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation there is no detectable phosphatase activity as determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.