T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5´ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.
Product SourceAn E. coli strain that carries a plasmid with the T5 phage D15 gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 4 -20 10X
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