Degradation of linear ssDNA, linear dsDNA and nicked plasmid DNA while preserving supercoiled plasmid DNA
Used in Gibson Assembly® method
T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5´ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.
Product SourceAn E. coli strain that carries a plasmid with the T5 phage D15 gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 4 -20 10X
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