Therminator™ γ DNA Polymerase

  • This product was discontinued on 01/01/2013
recombinant unique buffer heat inactivation no
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Description

Therminator γ (gamma) DNA Polymerase is a 9°N™ DNA polymerase variant with an enhanced ability to incorporate gamma-phosphate labeled dNTPs.

Product Source

Therminator γ DNA Polymerase is purified from a strain of E. coli that carries the 9°N (D141A / E143A / W355A / L408W / R460A / Q461S / K464E / D480V / R484W / A485L) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus species 9°N-7. Developed by LI-COR® Biosciences and derived from Therminator.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Therminator™ γ Reaction Buffer Pack-2010X

Advantages and Features

Applications

  • Incorporation of modified nucleotides into DNA
  • DNA sequencing using gamma-phosphate modified nucleotides

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Therminator™ γ Reaction Buffer Pack

1X Therminator™ γ Reaction Buffer Pack:
20 mM Tris-HCl
50 mM KCl
5 mM MgSO4
0.02% IGEPAL® CA-630
pH 9.2 @ 25°C

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
300 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 90000 daltons

5' - 3' Exonuclease

No

3' - 5' Exonuclease

No

Strand Displacement

+

Unit Assay Conditions

1X Therminator γ Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13mp18.

Tech Tips

Choose Therminator γ DNA Polymerase (#M0334) for incorporation of gamma-phosphate labeled dNTPs.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Troubleshooting Guides

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Exonuclease Activity (Radioactivity Release):
    The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.