Crimson Taq DNA Polymerase

An alternative product for purchase is M0486.
  • This product was discontinued on 01/05/2015
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Description

  
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

Crimson Taq DNA Polymerase offers superior performance in its newly formulated PCR buffer. Crimson Taq Reaction Buffer contains a density reagent, which allows direct loading of PCR products onto a gel. In addition, Crimson Taq Reaction Buffer has a trace amount of a red dye, which serves as an indicator for homogenous reaction setup, a color aid in gel loading and a tracking dye which migrates at about 10 bp on a 1% TBE gel.

Amplification of specific sequences from human genomic DNA using Crimson Taq DNA Polymerase. Amplicon sizes are indicated below gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Crimson Taq Reaction Buffer Pack-205X

Advantages and Features

Features

  • Robust and reliable reactions
  • Samples can be loaded directly onto a gel
  • Ideal for high throughput applications

Applications

  • PCR
  • Primer Extension
  • Colony PCR
  • High-throughput PCR

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 

Reaction Conditions

1X Crimson Taq Reaction Buffer Pack

1X Crimson Taq Reaction Buffer Pack:
12.5 mM Tricine
42.5 mM KCl
1.5 mM MgCl2
6% Dextran
0% Acid Red
pH 8.5 @ 25°C

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 94000 daltons

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

+1

Resulting Ends

  • Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Error Rate

~ 285x10-6bases

Notes

  1. 5'→3' flap endonuclease destroys displaced strand.

References

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
  4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res. . 18, 7317-7322.
  5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
  8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

FAQs

  1. What are the advantages or disadvantages of Crimson Taq DNA Polymerase?
  2. Does the 5X Crimson Taq Reaction Buffer offer amplification efficiency similar to that of Standard Taq Reaction Buffer or ThermoPol Reaction Buffer?
  3. How do I remove the dye and Dextran from my PCR reactions using Crimson Taq Reaction Buffer?
  4. How long is the Crimson Taq Reaction buffer stable under sub-optimal storage conditions?
  5. Can Taq DNA Polymerase be used in other buffers?
  6. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  7. Why is the product a smear when visualized on an agarose gel?
  8. Why is there no product when visualized on an agarose gel?
  9. The product sequence doesn't completely match the expected sequence. How can this result be improved?
  10. Does the presence of Ca2+ inhibit PCR?
  11. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
  12. Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  13. Can Taq DNA Polymerase be used for nick translation?
  14. How should I set up an amplification reaction using Crimson Taq DNA Polymerase?

Tech Tips

Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

Protocols

  1. PCR Protocol for Crimson Taq DNA Polymerase (M0324)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Troubleshooting Guides

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • PCR Amplification (Master Mix):
    The polymerase master mix is tested in a polymerase chain reaction (PCR) using a control template and specific primers, resulting in the expected product.
  • Single Stranded DNase Activity (FAM Labeled Oligo):
    The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide. The percent degradation is determined by capillary electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.