p19 siRNA Binding Protein
Avoiding RNase Contamination
The p19 siRNA Binding Protein (19 kDa) from the Carnation Italian Ringspot Virus (CIRV) plant binds siRNAs with nanomolar affinity(1). The dimeric protein binds 21 base siRNAs with a 2 base 3´ extension and a 5´phosphate. The protein binds RNA in a size dependent and sequence independent manner. If the siRNAs are 4 bases longer, the affinity for the protein is reduced about 100-fold (2). When p19 siRNA Binding Protein is expressed in plants it suppresses RNA interference (3).
- For the isolation of siRNA
- Isolated from a recombinant source
Product Sourcep19 siRNA Binding Protein is cloned and expressed in E. coli as a fusion protein with an amino terminal MBP (maltose binding protein) and a carboxy terminal CBD (chitin binding domain).
The following reagents are supplied with this product:
Store at (°C) Concentration p19 siRNA Binding Buffer (200 μl) -20 2 X
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