Unit Definition

One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg DNA Gyrase (E. coli ) Substrate to >95% supercoiled plasmid in a total reaction volume of 30 µl in 30 minutes at 37°C.

Reaction Conditions

1X DNA Gyrase (E. coli) Reaction Buffer
Incubate at 37°C

1X DNA Gyrase (E. coli) Reaction Buffer
35 mM Tris-HCl
24 mM KCl
4 mM MgCl₂
2 mM DTT
1.75 mM ATP
5 mM spermidine
0.1 mg/ml BSA
6.5% Glycerol
(pH 7.5 @ 25°C)

Storage Temperature

-80°C

Storage Conditions

10 mM Tris-HCl
50 mM KCl
2 mM DTT
50% Glycerol
0.1 mM EDTA
pH 7.5 @ 37°C

Heat Inactivation

65°C for 20 min

Supplied As

Liquid