DNA Gyrase (E. coli)

Companion Products

• Topoisomerase I (E. coli)

1. The 5X DNA Gyrase Reaction Buffer should be stored at -80°C to maintain optimal stability of components.

1. Adachi, T. et al. (1987). Nucleic Acids Res. 15, 771-784.
2. Higgins, N.P. et al. (1978). PNAS. 75, 1773-1777.
3. Swanberg, S.L. and Wang, J.C. (1987). J. Mol. Biol. 197, 729-736.

1. Is the linking number (ΔLk) or the superhelical density (σ) that results from the gyrase reaction fixed or random? Does the sequence, GC content or size of the circular DNA affect the linking number?
2. Will E. coli DNA Gyrase add additional negative supercoils in already supercoiled DNA?
3. Do you offer a reverse gyrase?
4. How should a DNA treated with gyrase be run on a gel?
5. Can E. coli DNA Gyrase supercoil close and open circular DNA?

1. Standard E. coli DNA Gyrase Reaction (M0306)

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0306S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0306S_L_v1_0021609
• M0306S_L_v1_0021701

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• DNA Gyrase (E. coli)
• DNA Gyrase (E. coli) Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.