9°Nm™ DNA Polymerase
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  • Catalog # M0260 was discontinued on January 01, 2013 ;
2019-20_Catalog_Promo_0519
  • Product Information

    9°Nm™ DNA Polymerase is a thermophilic DNA polymerase that has been genetically engineered to have a decreased 3'→ 5' proofreading exonuclease activity (1-5% of the wildtype). 9°Nm DNA Polymerase features a half-life of 6.7 hours at 95°C.

    9°Nm DNA Polymerase crystals (Ira Schildkraut and Rebecca Kucera, New England Biolabs, Inc.)

    Highlights

    • Isolated from a recombinant source
    • Supplied with 10X Reaction Buffer

    Product Source

    An E. coli strain that carries the 9°N (E143D) DNA Polymerase gene, (1,2) a genetically engineered form of the native DNA polymerase from the extremely thermophilic marine archaea Thermococcus species 9°N-7. The archaea was isolated from a submarine thermal vent, at a depth of 2,500 meters, 9° north of the equator at the East Pacific Rise (3).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    ThermoPol® Reaction Buffer Pack -20 10 X
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