Deep Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Deep Vent DNA Polymerase. Deep Vent (exo-) DNA Polymerase is even more stable than Vent® (exo-) DNA Polymerase with a half-life of 23 hours at 95°C and 8 hours at 100°C. Both Deep Vent (exo-) and Vent (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.
Product SourceAn E. coli strain that carries the Deep Vent (D141A / E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2,010 meters (1) and is able to grow at temperatures as high as 104°C.
The following reagents are supplied with this product:
Store at (°C) Concentration Magnesium Sulfate (MgSO4) Solution -20 100 mM ThermoPol® Reaction Buffer Pack -20 10X
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