Vent® (exo-) DNA Polymerase
Vent® DNA Polymerase offers moderate fidelity and robust performance
- No 3´→ 5´ proofreading exonuclease activity
- 2X higher fidelity than Taq
- Difficult templates: ideal for GC-rich or looped sequence
- High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Vent DNA Polymerase (1). This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase (2,3).
Product SourceAn E. coli strain that carries the Vent (D141A / E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus litoralis (4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
The following reagents are supplied with this product:
Store at (°C) Concentration Magnesium Sulfate (MgSO4) Solution -20 100 mM ThermoPol® Reaction Buffer Pack -20 10X
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