Vent® (exo-) DNA Polymerase
Vent® DNA Polymerase offers moderate fidelity and robust performance
- No 3´→ 5´ proofreading exonuclease activity
- 2X higher fidelity than Taq
- Difficult templates: ideal for GC-rich or looped sequence
- High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Vent DNA Polymerase (1). This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase (2,3).
Product SourceAn E. coli strain that carries the Vent (D141A / E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus litoralis (4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
The following reagents are supplied with this product:
Store at (°C) Concentration Magnesium Sulfate (MgSO4) Solution -20 100 mM ThermoPol® Reaction Buffer Pack -20 10 X
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