Vent® DNA Polymerase
Vent® DNA Polymerase offers moderate fidelity and robust performance
- Possesses 3´→ 5´ proofreading exonuclease activity
- 5X higher fidelity than Taq
- Difficult templates: ideal for GC-rich or looped sequence
- High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2). This high fidelity derives in part from an integral 3'→5' proofreading exonuclease activity in Vent DNA Polymerase (1,3). Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C.
Product SourceAn E. coli strain that carries the Vent DNA Polymerase gene from Thermococcus litoralis (4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
The following reagents are supplied with this product:
Store at (°C) Concentration Magnesium Sulfate (MgSO4) Solution -20 100 mM ThermoPol® Reaction Buffer Pack -20 10 X
- Product Categories:
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.