hOGG1 (α isoform) is an 8-oxoguanine DNA glycosylase which acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3´ to the AP site leaving a 5´ phosphate and a 3´-phospho-α, β-unsaturated aldehyde.
Some of the damaged bases recognized and removed by hOGG1 include 7, 8-dihydro-8-oxoguanine (8-oxoguanine) when base paired with cytosine, 8-oxoadenine when base paired with cytosine, foramidopyrimidine (fapy)-guanine and methy-fapy-guanine (1,2).
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Product SourceAn E. coli strain that carries the cloned human ogg1 gene (3).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10 X Purified BSA -20 10 mg/ml
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