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    Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab

    Description

    Amino terminal (N-terminal) of human Dnmt1 has been shown to bind proliferating cell nuclear antigen (PCNA) and histone deactylase (1,2). Deletion of this region stimulates DNA methylation (3). In neoplastic transformed cells methylation pattern is abnormal, leading to the speculation that Dnmt1 may be involved in such processes (4).

    Sensitivity:
    Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Antibody detects less than 10 ng of Dnmt1 in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology).

    Dnmt1 Amino-terminal Antibody:

    Polyclonal antibodies are produced by immunizing rabbit with a KLH coupled synthetic peptide corresponding to residues in the N-terminal region of human Dnmt1. The antibodies are purified in a protein A column.

    Figure 1: Western analysis of cell extracts; Lane 1: Sf9 cells, Lane 2: Human DNA (cytosine-5) Methyltransferase expressing Sf9 cells, Lane 3: 293 cells, Lane 4: Hela cells, Lane 5: Jurkat cells, Lane 6: COS cells, Lane 7: Mouse DNA (cytosine-5) Methyltransferase expressing Sf9 cells, using Human DNA
    Storage Conditions:
    10 mM HEPES
    150 mM NaCl
    100 µg/ml BSA
    50% Glycerol
    pH 7.5 @ 25°C

    Properties and Usage

    Antibody Type

    Polyclonal

    • Human
    • Monkey

    Recommended Dilution for Western Blot

    1:2000

    Storage Temperature

    -20°C

    References

    1. Chuang, L.S. et al. (1997). Science. 277, 1996-2000.
    2. Fuks, F. et al. (2000). Nature Genet. . 24, 88-91.
    3. Bestor, T.H. (1992). EMBO J. . 11, 2611-2617.
    4. Schmutte, C. et al. (1998). Biol. Chem. . 379, 377-388.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What dilution should be used with Dnmt1 Amino-terminal Ab?
    2. How much protein should be run on the gel for detection with Dnmt1 Amino-terminal Ab?
    3. How much of NEB Dnmt1 should be used as a control when using Dnmt1 Amino-terminal Ab?
    4. What is the species cross reactivity of Dnmt1 Amino-terminal Ab?
    5. Can Dnmt1 Amino-terminal Ab be used to detect mouse and rat?