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Home Discontinued Products Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab

Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab

Catalog # M0231 was discontinued on January 05, 2015 ;

Product Information
Amino terminal (N-terminal) of human Dnmt1 has been shown to bind proliferating cell nuclear antigen (PCNA) and histone deactylase (1,2). Deletion of this region stimulates DNA methylation (3). In neoplastic transformed cells methylation pattern is abnormal, leading to the speculation that Dnmt1 may be involved in such processes (4).

Sensitivity:
Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Antibody detects less than 10 ng of Dnmt1 in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology).

Dnmt1 Amino-terminal Antibody:

Polyclonal antibodies are produced by immunizing rabbit with a KLH coupled synthetic peptide corresponding to residues in the N-terminal region of human Dnmt1. The antibodies are purified in a protein A column.

Figure 1: Western analysis of cell extracts; Lane 1: Sf9 cells, Lane 2: Human DNA (cytosine-5) Methyltransferase expressing Sf9 cells, Lane 3: 293 cells, Lane 4: Hela cells, Lane 5: Jurkat cells, Lane 6: COS cells, Lane 7: Mouse DNA (cytosine-5) Methyltransferase expressing Sf9 cells, using Human DNA

Storage Conditions:
10 mM HEPES
150 mM NaCl
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Product Categories:

Discontinued Products
- Properties & Usage
  Antibody Type
  Polyclonal
  
  Human
  
  Monkey
  
  Recommended Dilution for Western Blot
  1:2000
  Storage Temperature
  -20°C
- Recommended Applications
  
  Detection Method
  Western Blot
  Sensitivity
  10.00
- Related Products
  Companion Products
  
  Human DNA (cytosine-5) Methyltransferase (Dnmt1)
- References
  Chuang, L.S. et al. (1997). Science. 277, 1996-2000.
  
  Fuks, F. et al. (2000). Nature Genet. . 24, 88-91.
  
  Bestor, T.H. (1992). EMBO J. . 11, 2611-2617.
  
  Schmutte, C. et al. (1998). Biol. Chem. . 379, 377-388.
Protocols, Manuals & Usage
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0231Datasheet-Lot0011205
  
  M0231Datasheet-Lot0011303
  
  M0231Datasheet-Lot0011403
FAQs & Troubleshooting
- FAQs
  What dilution should be used with Dnmt1 Amino-terminal Ab?
  
  How much protein should be run on the gel for detection with Dnmt1 Amino-terminal Ab?
  
  How much of NEB Dnmt1 should be used as a control when using Dnmt1 Amino-terminal Ab?
  
  What is the species cross reactivity of Dnmt1 Amino-terminal Ab?
  
  Can Dnmt1 Amino-terminal Ab be used to detect mouse and rat?
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0231Datasheet-Lot0011205
  
  M0231Datasheet-Lot0011303
  
  M0231Datasheet-Lot0011403
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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