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  • Human DNA (cytosine-5) Methyltransferase (Dnmt3A) Amino-terminal Ab

    Discontinued Date

    01/01/2013
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    Categories:
    Discontinued Products

    Description

    Dnmt3A is a de novo mammalian methyltransferase that is architecturally similar to Dnmt1 and Dnmt3B (1). The amino terminus (N-terminus) of human Dnmt3A contains various motifs that recruit transcriptional repressors (2). Dnmt3A cooperates with other DNA methyltransferases to maintain DNA methylation (3). Dnmt3A null embryos show multiple developmental defects including growth impairment and neural tube formation (4). Overexpression of DNA methyltransferases are observed in acute and chronic myelogenous leukemia cells (5). The apparent molecular weight for Dnmt3A is 101.5 kDa. 

    Sensitivity:

    Human DNA (cytosine-5) Methyltransferase (Dnmt3A) amino-terminal antibody detects less than 20 ng of Dnmt3A in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology).

    Figure 1: Western analysis of cell extracts using Human DNA (cytosine-5) Methyltransferase 3A Amino-Terminal Antibody; Lane 1: HeLa cells, Lane 2: A549 cells, Lane 3: A673 cells, Lane 4: 3T3 cells, Lane 5: C6 cells.
    Storage Conditions:
    10 mM Sodium HEPES
    150 mM NaCl
    100 µg/ml BSA
    50% Glycerol
    pH 7.5 @ 25°C

    Advantages and Features

    Applications

    Western Blotting

    Properties and Usage

    • Human
    • Rat
    • Mouse
    • Monkey

    Recommended Dilution for Western Blot

    1:2000

    Storage Temperature

    -20°C

    References

    1. Okano, M. et al. (1998). Nature Genet.. 19, 219-220.
    2. Pradhan, S. et al. (2003). Clinical Immunology . 109, 6-16.
    3. Kim, G. D. et al. (2002). EMBO J. 21, 4183-4195.
    4. Okano, M. et al. (1999). Cell. 99, 247-257.
    5. Mizuno, S. et al (2001). Blood. 97, 1172-1179.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.