HEN1 miRNA Methyltransferase

  • This product was discontinued on 01/01/2016
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Ordering Information

M0228
  • Discontinued
    Discontinued
  • Product Information
    HEN1 is a plant miRNA Methyltransferase that methylates the terminal ribose in short double-stranded RNAs. In Arabidopsis, 2´-0-methylation of the 3´ ribose of miRNAs and siRNAs is mediated by HEN1 (1, 2). In HEN1 mutants, small RNAs were found unmethylated with a 3´ poly (U) tail, suggesting 3´ end methylation protects small RNAs from uridylation (3). Purified HEN1 methylates both miRNA/miRNA and siRNA/siRNA duplexes with a preference for 21-24 nucleotide RNA duplexes with 2 nucleotide overhangs (4). HEN1 does not methylate single-stranded RNA or DNA.

    Product Source

    An E. coli strain that carries the cloned HEN1 from Arabidopsis.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBuffer™ 2 -20 10 X
    S-adenosylmethionine (SAM) -20 32 mM
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