HEN1 miRNA Methyltransferase
- This product was discontinued on 01/01/2016
HEN1 is a plant miRNA Methyltransferase that methylates the terminal ribose in short double-stranded RNAs. In Arabidopsis, 2´-0-methylation of the 3´ ribose of miRNAs and siRNAs is mediated by HEN1 (1, 2). In HEN1 mutants, small RNAs were found unmethylated with a 3´ poly (U) tail, suggesting 3´ end methylation protects small RNAs from uridylation (3). Purified HEN1 methylates both miRNA/miRNA and siRNA/siRNA duplexes with a preference for 21-24 nucleotide RNA duplexes with 2 nucleotide overhangs (4). HEN1 does not methylate single-stranded RNA or DNA.
Product SourceAn E. coli strain that carries the cloned HEN1 from Arabidopsis.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10 X S-adenosylmethionine (SAM) -20 32 mM
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