The lots provided in the NEBNext® Magnesium RNA Fragmentation Module (NEB #E6150) and the RNase III RNA Fragmentation Module (NEB #E6146) and the NEBNext® Second Strand Synthesis Module (NEB #E6111) are managed separately and are qualified by additional functional validation. Individual reagents meet stringent criteria in the additional quality controls listed on each individual component page.
The NEBNext® Magnesium RNA Fragmentation Module is functionally validated through construction of a transcriptome library using the NEBNext® mRNA sample preparation reagents and sequencing on an Illumina® sequencing instrument.
The NEBNext® Second Strand Synthesis Module has been validated by sequencing with an Illumina® Sequencer in conjunction with M-MuLV Reverse Transcriptase (RNase H-) (NEB #M0368), NEBNext® First Strand Synthesis Reaction Buffer, Murine RNase Inhibitor (NEB #M0314), the NEBNext® End Repair Enzyme Module (NEB #E6050), the NEBNext® dA-Tailing Module (NEB #E6053), the NEBNext® Quick Ligation Module (NEB #E6056), NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
NEBNext® is a registered trademark of New England Biolabs, Inc.
SuperScript® is a registered trademark of Invitrogen Corporation.
Phusion® is a registered trademark of Finnzymes Oy, a Thermo Fisher Scientific company.