NEB offers a variety of modules for use in the preparation of RNA for the leading sequencing platforms:
NEBNext® Poly(A) mRNA Magnetic Isolation Module
NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) is optimized to isolate intact poly(A)+ RNA from previously isolated total RNA. The module couples Oligo d(T)25 and 1 μm paramagnetic beads for the direct binding of poly(A)+ RNA. The procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent.
NEBNext® Magnesium RNA Fragmentation Module
NEBNext® Magnesium RNA Fragmentation Module (NEB #E6150) is optimized to fragment RNA into small pieces using divalent cations under elevated temperature. The module contains buffers that are suited for RNA fragmentation.
NEBNext® RNase III RNA Fragmentation Module
NEBNext® RNase III RNA Fragmentation Module (NEB #E6146) is optimized to fragment RNA into small pieces using RNase III enzyme. RNase III is an endonuclease enzyme that cleaves long double-stranded RNA into short fragments containing 5´-PO4 and 3´-OH ends. The module contains enzyme and buffer that are suited for RNA fragmentation.
NEBNext® Second Strand Synthesis Module
NEBNext® Second Strand Synthesis Module (NEB #E6111) is optimized to generate double stranded cDNA from first strand cDNA. The module is provided as a master mix to maximize efficiency and convenience in RNA sample preparation workflows. The dsDNA generated by the NEBNext Second Strand Synthesis Module
(NEB #E6111) can be subsequently converted to blunt-ended DNA fragments using the NEBNext End Repair Module (NEB #E6050).