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  • RNA Master Mix Sets

    NEB offers mRNA Library Preparation Master Mix Sets for the following platforms:

    Each set contains enzymes and buffers that are ideally suited for library preparation for next generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Sets are also available for the preparation of small RNA for the following platforms:

    Each NEBNext® Small RNA Library Prep Set contains adaptors, primers, enzymes and buffers that are ideally suited to convert small RNA transcripts into cDNA libraries (barcoded libraries for multiplex Illumina) for next generation sequencing on the Illumina platform . Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

    Illumina® is a registered trademark of Illumina, Inc.
    454™ is a trademark of Roche.
    SOLiD™ is a trademark owned by Life Technologies, Inc.
    • A Breakthrough Method of RNA Sample Preparation

      The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

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    Advantages of NEBNext Reagents for RNA Library Preparation

    • Convenient Formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available. 
    • Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, followed by sequencing on the appropriate sequencing platform (Illumina, 454 or SOLiD). 
    • Stringent Quality Controls 
    • Value Pricing 
    • Novel Protocol for small RNA – Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs

    Applications of NEBNext Reagents for RNA Library Preparation

    • mRNA library preparation for next generation sequencing
    • Small RNA library preparation for sequencing with Illumina and SOLiD
    • Expression library construction

    mRNA Library Construction Workflow

    mRNA Library Construction Workflow for Illumina

    mRNA Library Construction Workflow for 454

    mRNA Library Construction Workflow for SOLiD

    Small RNA Library Construction Workflow

    Small RNA Library Construction Workflow for Illumina

    Small RNA Library Construction Workflow for SOLiD