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  • Library Preparation for Next Generation Sequencing

    NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximized flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

    Each set of reagents is functionally validated together through construction of a library from a DNA or RNA reference sample that is then sequenced on the appropriate platform. Whenever a new lot of any of the reagents is introduced into a kit, the entire kit is re-validated by library construction and sequencing.

    NEBNext reagents are available for sample preparation for Illumina®, Ion Torrent™ and 454™ sequencing platforms.

    NEBNext® is a registered trademark of New England Biolabs, Inc.
    Illumina® is a registered trademark of Illumina, Inc.
    Ion Torrent™is a trademark of Life Technologies, Inc.

    454™ is a trademark of Roche.

    1. A Breakthrough Method of Small RNA Sample Preparation

      The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

    2. NEBNext Direct® Target Enrichment

      Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.

    3. NEBNext Ultra II DNA Library Prep Protocol

      This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

    Featured Products

    Library Preparation for Next Generation Sequencing includes these subcategories:

    DNA Master Mix Sets Information
    DNA Reagent Sets Information
    DNA Modules Information
    DNA Enzymes Information
    RNA Master Mix Sets Information
    RNA Reagent Sets Information
    RNA Modules Information

      Publications related to Library Preparation for Next Generation Sequencing:

    1. Luck, A.N., Anderson, K.G., McClung, C.M., VerBerkmoes, N.C., Foster, J.M., Michalski, M.L. and Slatko, B.E. (2015). Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont BMC Genomics. 12864-015-2083-2. PubMedID: 26559510


    • Convenient Formats – All of the required enzymes, buffers and nucleotides are included in our sets and modules; many are available in master mix format. Sets for Small RNA and for Ion Torrent include adaptors and primers. Modules offer the ability to customize sample preparation.
    • Functional Validation – Each reagent set or module is functionally validated by preparation of a genomic DNA library that is then sequenced using the appropriate sequencing platform (Illumina, Roche/454, SOLiD or Ion Torrent) and by preparation of an expression library or single-stranded DNA or by preparation of a library from an accepted RNA reference sample.
    • Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
    • Value Pricing