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  • DNA Master Mix Sets

    NEB offers DNA Library Preparation Master Mix Sets for the following protocols/platforms:

    Master Mix Sets are also available for the preparation of ChIP-Seq DNA for the Illumina platform (NEB #E6240).

    Each set contains enzymes and buffers in convenient master mix formulations that are ideally suited for library preparation for next generation sequencing of genomic or Chromatin Immunoprecipitated (ChIP) DNA. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
    Illumina® is a registered trademark of Illumina, Inc.
    454™ is a trademark of Roche.
    Ion Torrent™ is a trademark owned by Life Technologies, Inc.
    1. 12 Quick Tips for NGS Library Preparation

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    2. Which NEBNext Product Format Should I Choose?

      Not sure how sets and kits differ from modules? Ping explains the difference, and helps you to decide which one is right for you.

    3. Tips for Optimizing DNA Inputs in NGS Library Construction

      Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

    4. Tips for Bead-based Clean ups and Size Selection

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    Advantages of NEBNext Reagents for DNA Library Preparation

    • Convenient Formats – All of the required enzymes, buffers and nucleotides are included, many available in master mix format. Sets for Ion Torrent include adaptors and primers. Modules offer the ability to customize sample preparation.
    • Functional Validation – Each reagent set or module is functionally validated by preparation of a genomic DNA or ChIP-Seq DNA library that is sequenced using the appropriate sequencing platform (Illumina, 454, SOLiD or Ion Torrent).
    • Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
    • Value Pricing

    Applications of NEBNext Reagents for DNA Library Preparation

    • Next generation sequencing library preparation
    • Expression library construction
    • High density hybridization arrays 
    • Genomic subtraction hybridization methods

    DNA Library Construction Workflow

    DNA Library Construction Workflow for Illumina

    Quick DNA Library Construction Workflow for 454

    DNA Library Construction Workflow for 454

    DNA Library Construction Workflow for SOLiD

    DNA Library Construction Workflow for Ion Torrent

    ChIP-Seq Library Construction Workflow