sgRNA Synthesis Reagents and Kits

Cas9 experiments require the introduction of guide RNAs, in addition to Cas9 nuclease. sgRNAs contain a 20-base sequence, specific to the target DNA, upstream of an invariant scaffold sequence. sgRNAs can be delivered as an RNA made in vitro, or by delivering an expression cassette in which the sgRNA is transcribed from an upstream promoter. For users who already have a template, NEB’s HiScribe kits can be used for generating microgram quantities of sgRNA. For Spy Cas9 users without a template, the EnGen sgRNA Synthesis Kit (NEB #E3322) provides a rapid workflow for generating guide RNA using a single user-supplied oligonucleotide.

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sgRNA Synthesis Reagents and Kits

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