sgRNA Synthesis Reagents and Kits
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sgRNA Synthesis Reagents and Kits
- Is it necessary to add a “G” into the oligo following the T7 promoter sequence?
- The yield of my sgRNA is low – what can I do to improve the yield?
- Is it necessary to DNase treat my sgRNA synthesis reaction?
- Why is my sgRNA not exhibiting 100% in vitro Cas9 cleavage activity?
- Is it necessary to phosphatase treat my sgRNAs?
- The sgRNA is not running as a single band on the gel – why is this happening?
- Will the sgRNAs synthesized from this kit (NEB #E3322S) be recognized by homologs of Cas9 from different organisms?
- Do I include the PAM(NGG) in my sgRNA sequence?
- What is the sequence of the control oligo provided with the EnGen sgRNA Synthesis Kit, S. pyogenes?
- Is the Monarch RNA Cleanup Kit (NEB #T2040) compatible with the EnGen sgRNA Synthesis Kit, S. pyogenes?
- Is it necessary to add the provided DTT to the sgRNA synthesis reaction?
- Does NEB provide plasmids for sgRNA cloning?
sgRNA Synthesis Reagents and Kits
- EnGen® sgRNA Synthesis Kit, S. pyogenes Protocol (E3322)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- sgRNA Synthesis Using the HiScribe™ Quick T7 High Yield RNA Synthesis Kit (NEB #E2050)
- How to synthesize a sequence-specific sgRNA for experiments using EnGen® Sau Cas9
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CRISPR/Cas9 & Targeted Genome Editing: New Era in Molecular Biology
Understand the history, importance and future of CRISPR/Cas9 and target genome editing
- Genome Editing Brochure
- RNA Synthesis Brochure
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