Mutation Detection Reagents and Kits

A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay. This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection Kit (NEB #E3321) provides optimized reagents for performing robust T7 Endonuclease-based detection of genome editing events. Additionally, NEB carries a broad range of next-generation sequencing kits for further analysis of editing events.

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