- Can cell lysates be used in the PCR reaction?
- My PCR reaction yield is low, can I add more than 5 µl of the PCR reaction to the digestion reaction?
- What are the expected results using the included control?
- Why do I see an extra band when I run the undigested heteroduplex on an agarose gel?
- Why do I see very little DNA on my gel or low signal on the fragment analyzer?
- Will EnGen® T7 Endonuclease I recognize single base mismatches and single base insertions or deletions (indels)?
CRISPR/Cas9 & Targeted Genome Editing: New Era in Molecular Biology
Understand the history, importance and future of CRISPR/Cas9 and target genome editing
- Genome Editing Brochure
- RNA Synthesis Brochure
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For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.
In this webinar you will learn how to increase editing efficiency by directly introducing Cas9 ribonucleoproteins (RNPs) to cells through electroporation or lipofection. Rapid sgRNA synthesis requiring only a single user-supplied ~55mer single-stranded DNA oligonucleotide is described. Methods for assessing genome editing efficiency will be discussed including T7-endonuclease I-based methods, sequencing-based methods, and in vitro Cas9 digestion.