Cypridina Luciferase (CLuc), from the marine ostracod Cypridina noctiluca is efficiently secreted from mammalian cells (1,2). CLuc does not react with coelenterazine, a common substrate of marine luciferases. It uses a specific luciferin, which is not a substrate for Gaussia Luciferase. This allows the detection of both of GLuc and CLuc expressed from the same cells.
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