DNA methyltransferases are enzymes that modify DNA by adding a methyl group to either cytosines or adenines depending on the specificity of the enzyme. DNA methyltransferases can be used experimentally to methylate DNA at specific sites for gene expression studies. Our selection includes CpG methyltransferase (NEB #M0226), which is especially useful for studying CpG methylation effects. These enzymes are also useful for producing positive controls for methylation-specific PCR or bisulfite sequencing.
Several protein methyltransferases (G9A (NEB #M0235), SET7 (NEB #M0233), PRMT1 (NEB #M0234)) are also available for the methylation of specific lysine and arginine residues in Histone H3 and H4 in gene regulation studies.
FAQs for Methyltransferases for Epigenetics
- What should be considered if the methylation using SssI Methyltransferase is not going to completion?
- How does Dnmt1 differ from SssI methylase?
- Can SssI Methyltransferase single stranded DNA?
- Can SssI Methyltransferase be used for generating a positive control for methylation-specific PCR or bisulfate sequencing?
- What is the molecular weight of SssI (CpG) Methyltransferase?
- Will all the sites in the DNA become methylated by SssI Methyltransferase?
- Can DNA be radiolabeled with SssI Methyltransferase?
- Can Dnmt1 be heat inactivated?
- Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
- Can SssI methylated DNA be used to transform E. coli?
- Does SssI Methyltransferase require magnesium in the buffer?
- What is the activity of SssI Methyltransferase in other NEBuffers, including CutSmart?
- What source of tritiated SAM is recommended for use with Dnmt1?
- What is the specific activity of SssI Methyltransferase?
- What should be considered if the methylation using Dnmt1 is not going to completion?
- Positive controls for methylation specific PCR or bisulfite sequencing
- CpG-methylated gene expression studies
- Nucleosome footprinting
Types of Histone Modifications
Types of DNA Modifications
- Kim, J.K., Samaranayake, M. and Pradhan S. (2009) Cell. Mol. Life Sci. 66, 596-612. PMID: 18985277
- Vanyushin, B.F. (2006) Curr. Top. Microbiol. Immunol. 301, 67-122. PMID: 16570846
- Mosher, R.A., Melnyk, C.W. (2010) Trends Plant Sci. 15, 204-210. PMID: 20129810
- Lyko, F., Beisel, C., Marhold, J., Paro, R. (2006) Curr. Top. Microbiol. Immunol. 310, 23-44. PMID: 16909905
- Selker, E.U., Freitag, M., Kothe, G.O., et al. (2002) Proc. Natl. Acad. Sci. U S A. 99, Suppl 4, 16485-16490. PMID: 12189210
- Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-930. PMID: 19372393
- Tahiliani, M., Koh, K. P., Shen, Y., et al. (2009) Science 324, 930-935. PMID: 19372391
- Ehrlich, M., Wilson, G.G., Kuo, K.C., And Gehrke, C.W. (1987) J. Bacteriol. 169, 939-943. PMID: 3029036
- Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A. and Matvienko, N.I. (2004) Biochem. (Moscow) 69, 299-305. PMID: 15061697
- Ratel, D., Ravanat, J-L., Berger, F. and Wion D. (2006) Bioessays 28, 309-315. PMID: 16479578
- Marinus, M.G. and Casadesus, J. (2009) FEMS Microbiol. Rev. 33, 488-503. PMID: 19175412
If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.