Methylation-Sensitive Restriction Enzymes

Methylation of DNA is the most studied epigenetic modification. Many restriction enzymes are sensitive to the DNA methylation states. Cleavage may be blocked, or impaired, when a particular base in the enzyme's recognition site is modified.

Methylation-sensitive restriction enzymes important in epigenetics from NEB include:

DpnI (NEB #R0176) and DpnII (NEB #R0543) for N6-methyladenine detection within GATC recognition site
HpaII (NEB #R0171) and MspI (NEB #R0106) for C5-methylcytosine detection within CCGG recognition site

What Is Epigenetics?
If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Research Division Head, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.
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Other Tools & Resources

Other Tools & Resources

Brochures

Epigenetics Brochure

The Epigenetics brochure provides information on these unique solutions to study DNA and histone modifications. NEB offers a suite of validated products for epigenetics research.