Methylation Sensitive Restriction Enzymes for Epigenetics
Methylation of DNA is the most studied epigenetic modification. Many restriction enzymes are sensitive to the DNA methylation states. Cleavage may be blocked, or impaired, when a particular base in the enzyme's recognition site is modified.
Methylation sensitive restriction enzymes can be used to generate fragments for further epigenetic analysis. When used in conjunction with an isoschizomer that has the same recognition site, but is methylation insensitive, information about methylation status can be obtained. Examples of methylation-sensitive restriction enzymes important in epigenetics include:
- DpnI (NEB #R0176) and DpnII (NEB #R0543) for N6-methyladenine detection within GATC recognition site
- HpaII (NEB #R0171) and MspI (NEB #R0106) for C5-methylcytosine detection within CCGG recognition site
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Epigenetics - Expanding on Genomic Foundations
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Epigenetics Brochure
The Epigenetics brochure provides information on these unique solutions to study DNA and histone modifications. NEB offers a suite of validated products for epigenetics research.
- Dam-Dcm and CpG Methylation
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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What Is Epigenetics?
If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.
