Epigenetic Analysis (Epimark® Validated)

Epigenetics
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    The EpiMark® line of products from New England Biolabs is designed to simplify DNA methylation and hydroxymethylation detection and analysis, as well as ChIP, histone and nucleosome analysis. This growing line of reagents and kits includes:

    • EpiMark® Methylated DNA Enrichment Kit (NEB #E2600) to enrich double-stranded methyl-CpG from fragmented genomic DNA with as little as 5 ng of input DNA
    • EpiMark® Bisulfite Conversion Kit (NEB #E3318) to detect methylated cytosine via the conversion of unmodified cytosines to uracil
    • EpiMark® Hot Start Taq DNA Polymerase (NEB #M0490) to amplifiy bisulfite-treated samples
    • EpiMark® 5-mC and 5-hmC Analysis Kit (NEB #E3317) to identify and quantify 5-hydroxymethylcytosine at specific loci
    • EpiMark® Nucleosome Assembly Kit (NEB #E5350) to prepare recombinant nucleosomes
    • NEBNext® ChIP-Seq Library Prep Master Mix for Illumina® (NEB #E6240) and NEBNext® ChIP-Seq Library Prep for SOLiD™ (NEB #E6260) to to prepare Chromatin immunoprecipitateed DNA samples for next-generation sequencing

    EpiMark® and NEBNext® are registered trademarks of New England Biolabs, Inc.
    Illumina® is a registered trademark of Illumina, Inc.
    SOLiD™ is a trademark of Life Technologies, Inc.

    Videos

    1. What Is Epigenetics?

      If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

    Learn More

      Publications related to Epigenetic Analysis (EpiMark® Validated):

    1. McKernan KJ, Spangler J, Zhang L, Tadigotla V, McLaughlin S, Warner J, Zare A, Boles RG (2014). Expanded genetic codes in next generation sequencing enable decontamination and mitochondrial enrichment PLoS One. 9(5), e96492. PubMedID: 24788618, DOI: 10.1371/journal.pone.0096492

    Types of Histone Modifications

    Types of DNA Modifications

    References

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    2. Vanyushin, B.F. (2006) Curr. Top. Microbiol. Immunol. 301, 67-122. PMID: 16570846
    3. Mosher, R.A., Melnyk, C.W. (2010) Trends Plant Sci. 15, 204-210. PMID: 20129810
    4. Lyko, F., Beisel, C., Marhold, J., Paro, R. (2006) Curr. Top. Microbiol. Immunol. 310, 23-44. PMID: 16909905
    5. Selker, E.U., Freitag, M., Kothe, G.O., et al. (2002) Proc. Natl. Acad. Sci. U S A. 99, Suppl 4, 16485-16490. PMID: 12189210
    6. Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-930. PMID: 19372393
    7. Tahiliani, M., Koh, K. P., Shen, Y., et al. (2009) Science 324, 930-935. PMID: 19372391
    8. Ehrlich, M., Wilson, G.G., Kuo, K.C., And Gehrke, C.W. (1987) J. Bacteriol. 169, 939-943. PMID: 3029036
    9. Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A. and Matvienko, N.I. (2004) Biochem. (Moscow) 69, 299-305. PMID: 15061697
    10. Ratel, D., Ravanat, J-L., Berger, F. and Wion D. (2006) Bioessays 28, 309-315. PMID: 16479578
    11. Marinus, M.G. and Casadesus, J. (2009) FEMS Microbiol. Rev. 33, 488-503. PMID: 19175412

    ChIP-Seq Library Construction Workflow