Control DNAs are powerful tools in the investigation of genomic DNA methylation and epigenomic research. They can be utilized in many protocols where they can serve as comparisons to sample DNA. Both positive and negative methylation control DNA are available for bisulfite conversion methods, methyl-DNA immunoprecipitation (Me-DIP) or by methyl-CpG binding domain-based (MBDCap) proteins methylation-specific PCR (MSP) or real time PCR.
Protocols for Control DNA
- SNP analysis
- Southern blotting
- Genomic DNA library construction
- Methylation-specific PCR (MSP)
- Bisulfate sequencing
- Methylation-sensitive single nucleotide primer extension (ms-SNUPE)
- Combined bisulfite restriction analysis (COBRA)
- Bisulfite treatment and PCR Single/Stranded Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)
If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.