This system provides a powerful and unique tool to study the role of proteins in a variety of highly dynamic processes, including protein trafficking, turnover, and complex formation. The starter kit provides all the components necessary for the expression and labeling of the gene of interest in mammalian cells. The kit includes a mammalian expression plasmid encoding the SNAP-tag® flanked by restriction sites for cloning a gene of interest, and two cell-permeable fluorescent SNAP-tag substrates, in addition to positive and negative controls.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|pSNAPf Vector||-20||0.5 mg/ml|
|pSNAPf-Cox8A Control Plasmid||-20||0.5 mg/ml|
|SNAP-Cell® 505-Star||-20||10 nmol|
|SNAP-Cell® TMR-Star||-20||6 nmol|
|SNAP-Cell® Block||-20||20 nmol|
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