pMAL™ Protein Fusion and Purification System

This product has been replaced by the NEBExpress® MBP Fusion and Purification System (NEB #E8201), which offers enhanced performance.

  • Catalog # E8200 was discontinued on June 30, 2020

The pMAL™ Protein Fusion and Purification System requires a cloned gene be inserted into a pMAL vector down-stream from the malE gene, which encodes maltose-binding protein (MBP). This results in the expression of an MBP-fusion protein. The technique uses the strong Ptac promoter and the translation initiation signals of MBP to express large amounts of the fusion protein. The fusion protein is then purified by a one-step affinity purification specific for MBP.

  • Expression in either the cytoplasm or periplasm: periplasmic expression can enhance folding of proteins with disulfide bonds
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli
  • Gentle elution with maltose; no detergents or harsh denaturants required
  • Reliable expression and substantial yields (up to 100 mg/L)

Store Amylose Resin at 4°C and all other components at -20°C. See Notes for long term storage.

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