Anti-MBP Antiserum

This product has been discontinued. Anti-MBP Monoclonal Antibody (E8032S/L) is a suitable alternative for most applications.
  • This product was discontinued on 09/11/2013
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Discontinued Products

Description

Rabbit antiserum prepared by immunizing against affinity-purified maltose-binding protein.

Properties and Usage

Recommended Dilution for Western Blot

1/10,000

Storage Temperature

-20°C

Notes

  1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3.

Protocols

  1. Western Transfer Protocol for Anti-MBP Antiserum

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Assurance Statement

  • In an ELISA assay, a dilution of 1/10,000 added to a microtiter plate containing 1 µg purified maltose-binding protein per well gives a value of approximately 1.0 at OD490 after 8 minutes reaction time, using OPD as the substrate for horseradish peroxidase. The same dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. Once diluted, the serum may be reused a few times in Western blots.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.