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    Anti-GLuc Antibody

    Description

    Anti-GLuc Antibody is an IgG fraction from rabbit serum immunized with recombinant Gaussia luciferase (GLuc). It can detect GLuc secreted from cells or in cell lysates.

    Western blot with anti-GLuc Antibody of proteins expressed in HEK-293 cells. GLuc and a non-secreted GLuc-fusion protein (~50 kDa) were detected in cell culture media and cell lysates. Lane 1: Untransfected cells; Lanes 2&3: GLuc-fusion; Lane 4: GLuc. The gel was blotted onto PVDF membrane; GLuc Antibody was diluted 1:5000 and detected with a chemiluminescent substrate and HRP-coupled anti-rabbit secondary antibody.

    Properties and Usage

    Recommended Dilution for Western Blot

    1:1000-1:5000

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • The Anti-GLuc Antibody is used in a Western analysis detecting the presence of Gaussia Luficerase in the growth medium and in the cell lysate after transfection of cells (mammalian) with a GLuc-expressing vector.

    Supporting Documents

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What portion of the GLuc protein does the Anti-GLuc Antibody bind to?
    2. What is the concentration of the Anti-GLuc Antibody?
    3. What amount of growth media from cells expressing GLuc should I use to detect the presence of GLuc by using the Anti-GLuc Antibody in a Western?
    1. Western Analysis (E8023)

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