NEBNext® ChIP-Seq Library Prep Set for SOLiD™

  • This product was discontinued on 01/01/2013
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Description

The NEBNext ChIP-Seq Library Prep Set for SOLiD contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing of Chromatin Immunoprecipitated DNA. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the SOLiD™ 4.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext End Repair Reaction Buffer10X
Quick T4 DNA Ligase-20
NEBNext Quick Ligation Reaction Buffer-205X
NEBNext End Repair Enzyme Mix
LongAmp™ Taq 2X Master Mix-202X

Properties and Usage

Storage Temperature

-20°C

FAQs

  1. Are NEBNext® products validated in Next Generation Sequencing workflows?

Protocols

  1. NEBNext End Repair Module Protocol (E6260)
  2. Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
  3. Adaptor Ligation of End Repaired DNA (E6260)
  4. Purification of Adaptor Ligated DNA (E6260)
  5. Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
  6. Purification of Amplified DNA (E6260)
  7. Perform a Second Purification of the Amplified DNA (E6260)

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.