NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

Please note that for ChIP DNA we now recommend the new NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645 ).

Ordering Information

  • 12 reactions
  • 60 reactions
  • Product Information

    The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    ChIP DNA Library Preparation Workflow for Illumina
    ChIP DNA Library Preparation Workflow for Illumina

    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE) 2 X
    NEBNext dA- Tailing Reaction Buffer -20 10 X
    Klenow Fragment (3´→5´ exo-) -20
    Quick T4 DNA Ligase -20
    NEBNext End Repair Enzyme Mix -20
    NEBNext End Repair Reaction Buffer -20 10 X
    NEBNext Quick Ligation Reaction Buffer -20 5 X
    NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
    Product Categories:
    ChIP-Seq Library Prep
    DNA for Illumina®,
    DNA Sequencing
    Illumina® Library Preparation
    • Properties and Usage
    • Notes
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