Materials Required but not Supplied

• 80% Ethanol (freshly prepared)
• Nuclease-free Water
• 0.1X TE, pH 8.0
• 10 mM Tris-HCl, pH 7.5–8.0
• 10 mM NaCl (optional)
• DNA LoBind Tubes (Eppendorf #022431021)
• AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
• NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E6609 or #E7600)
• Magnetic rack/stand
• PCR Machine

1. Are NEBNext® products validated in Next Generation Sequencing workflows?
2. Are DNA adaptors or oligonucleotides included in the NEBNext® products?
3. How do I know the concentration of my index primers? 

1. Please see manual (NEB #E6240) for protocols

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)
• NEBNext dA- Tailing Reaction Buffer
• Klenow Fragment (3´→5´ exo-)
• Quick T4 DNA Ligase
• NEBNext End Repair Enzyme Mix
• NEBNext End Repair Reaction Buffer
• NEBNext Quick Ligation Reaction Buffer
• NEBNext® Q5® Hot Start HiFi PCR Master Mix

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

This product is covered by one or more Patents. 
This product is licensed from Bio-Rad Laboratories, Inc. under U.S. Pat. Nos. 6,627,424; 7,541,170; 7,670,808; 7,666,645 and corresponding patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not real-time PCR or digital PCR; (b) any in vitro diagnostics application, except for applications using real time or digital PCR; and (c) any non-PCR applications in DNA sequencing, isothermal amplification and the production of synthetic DNA. 

Trademarks

Notice to Customer: Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.
Notice to Customer: Limited License. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by Bio-Rad Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. This product is licensed from Bio-Rad Laboratories, Inc. under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not real time PCR or digital PCR; (b) any in vitro diagnostics application, except for applications using real time or digital PCR; and (c) any non-PCR applications in DNA sequencing, isothermal amplification and the production of synthetic DNA.

Phusion® is a registered trademark and property of Thermo Fisher Scientific.
Illumina® is a registered trademark of Illumina, Inc.

• This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.