NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®


The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

ChIP DNA Library Preparation Workflow for Illumina
ChIP DNA Library Preparation Workflow for Illumina

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext End Repair Reaction Buffer10X
Klenow Fragment (3´→5´ exo-)
NEBNext dA- Tailing Reaction Buffer10X
Quick T4 DNA Ligase-20
NEBNext Quick Ligation Reaction Buffer-205X
NEBNext End Repair Enzyme Mix
NEBNext® Q5® Hot Start HiFi PCR Master Mix-20
NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)2X

Properties and Usage

Materials Required but not Supplied

  • 80% Ethanol (freshly prepared)
  • Nuclease-free Water
  • 0.1X TE, pH 8.0
  • 10 mM Tris-HCl, pH 7.5–8.0
  • 10 mM NaCl (optional)
  • DNA LoBind Tubes (Eppendorf #022431021)
  • AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
  • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E6609 or #E7600)
  • Magnetic rack/stand
  • PCR Machine

Storage Temperature



  1. Are NEBNext® products validated in Next Generation Sequencing workflows?
  2. Are DNA adaptors or oligonucleotides included in the NEBNext® products?
  3. How do I know the concentration of my index primers? 


  1. Please see manual (NEB #E6240) for protocols


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.