NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®


The ChIP-Seq Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

ChIP DNA Library Preparation Workflow for Illumina
ChIP DNA Library Preparation Workflow for Illumina

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Phosphorylation Reaction Buffer-2010X
Deoxynucleotide Solution Mix10 mM
T4 DNA Polymerase
DNA Polymerase I, Large (Klenow) Fragment-20
T4 Polynucleotide Kinase
Deoxyadenosine 5´- Triphosphate (dATP)-201 mM
Klenow Fragment (3´→5´ exo-)
NEBuffer 2 for Klenow Fragment (3´→ 5´ exo)10X
Quick T4 DNA Ligase-20
Quick Ligation Reaction Buffer2X
NEBNext® Q5® Hot Start HiFi PCR Master Mix-20

Properties and Usage

Materials Required but not Supplied

  • 80% Ethanol (freshly prepared)
  • Nuclease-free Water
  • 0.1X TE, pH 8.0
  • 10 mM Tris-HCl, pH 7.5–8.0
  • 10 mM NaCl (optional)
  • DNA LoBind Tubes (Eppendorf #022431021)
  • AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
  • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7710, #E7730 or #E7600)
  • Magnetic rack/stand
  • PCR Machine

Storage Temperature



  1. For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact for further information.


  1. How do I know the concentration of my index primers? 


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.