NEBNext® Small RNA Library Prep Set for SOLiD™
- Catalog # E6160 was discontinued on January 19, 2016 ;
The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the SOLiD™ 4.
Recent article in GenomeWeb reviews NEBNext Small RNA Kits.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration T4 DNA Ligase Reaction Buffer -20 10 X Linear Acrylamide -20 10 mg/ml NEBNext 3' Ligation Reaction Buffer -20 2 X NEBNext 3´ Ligation Enzyme Mix -20 Murine RNase Inhibitor -20 NEBNext 5' Ligation Reaction Buffer -20 10 X NEBNext 5' Ligation Enzyme Mix -20 DNA Gel Elution Buffer 4 1 X TE Buffer -20 Nuclease-free Water -20 Gel Loading Dye, Blue 25 6 X NEBNext 3' SR Adaptor 3 -20 NEBNext 5' SR Adaptor 3 -20 NEBNext SR RT Primer 3 -20 NEBNext Index 1 Primer for SOLiD -20 NEBNext SR Primer R3 -20 Quick-Load pBR322 DNA-MspI Digest -20 100 µg/ml OneTaq Hot Start 2X Master Mix with Standard Buffer -20 2 X ProtoScript® II Reverse Transcriptase -20 200000 units/ml NEBNext First Strand Synthesis Reaction Buffer -20 5 X
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