NEBNext® Small RNA Library Prep Set for SOLiD™

1. Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)

1. Is Index 1 in the NEBNext Small RNA Library Prep Set for SOLiD kit (E6160) compatible with the NEBNext Small RNA Multiplex (1-16) Prep Set for SOLiD kit (E7450)?
2. If my input is <5ug and therefore use 0.5 ul of  3' SR Adapter 3, SR RT Primer 3 and 5' SR Adapter 3, instead of 1.0 ul, my total volumes for each reaction will be short by 0.5 ul. Should I replace that lost volume with water?
3. Why do I add the SR RT Primer 3 before the 5’ ligation instead of right before the reverse transcription step?
4. If I forget to add the 3’ SR RT Primer 3 before the 5’ ligation can I add it before the reverse transcription step and move forward?
5. Can samples be stored longer than 72 hours at -20°C?
6. How many cycles of PCR should I do?
7. If I accidentally move all 30 ul of the 5’ ligation product forward to the reverse transcription mixture will the reaction still work?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext® Small RNA Library Prep Set for SOLiD™
• T4 DNA Ligase Reaction Buffer
• Murine RNase Inhibitor
• DNA Gel Elution Buffer
• TE Buffer
• Gel Loading Dye, Blue
• NEBNext 3' SR Adaptor 3
• NEBNext SR RT Primer 3
• NEBNext Index 1 Primer for SOLiD
• NEBNext SR Primer R3
• OneTaq Hot Start 2X Master Mix with Standard Buffer
• ProtoScript® II Reverse Transcriptase

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Licenses

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer requires a license from New England Biolabs, Inc. Please contact [email protected] for more information.