NEBNext® Small RNA Library Prep Set for SOLiD™

1. Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)

1. Is Index 1 in the NEBNext Small RNA Library Prep Set for SOLiD kit (E6160) compatible with the NEBNext Small RNA Multiplex (1-16) Prep Set for SOLiD kit (E7450)?
2. If my input is <5ug and therefore use 0.5 ul of  3' SR Adapter 3, SR RT Primer 3 and 5' SR Adapter 3, instead of 1.0 ul, my total volumes for each reaction will be short by 0.5 ul. Should I replace that lost volume with water?
3. Why do I add the SR RT Primer 3 before the 5’ ligation instead of right before the reverse transcription step?
4. If I forget to add the 3’ SR RT Primer 3 before the 5’ ligation can I add it before the reverse transcription step and move forward?
5. Can samples be stored longer than 72 hours at -20°C?
6. How many cycles of PCR should I do?
7. If I accidentally move all 30 ul of the 5’ ligation product forward to the reverse transcription mixture will the reaction still work?

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The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext® Small RNA Library Prep Set for SOLiD™
• T4 DNA Ligase Reaction Buffer
• Murine RNase Inhibitor
• DNA Gel Elution Buffer
• TE Buffer
• Gel Loading Dye, Blue
• NEBNext 3' SR Adaptor 3
• NEBNext SR RT Primer 3
• NEBNext Index 1 Primer for SOLiD
• NEBNext SR Primer R3
• OneTaq Hot Start 2X Master Mix with Standard Buffer
• ProtoScript® II Reverse Transcriptase

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based OneTaq® Hot Start 2X Master Mix with Standard Buffer requires a license from New England Biolabs, Inc. Please contact [email protected] for more information.