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  • NEBNext® Small RNA Library Prep Set for Illumina®

    This product is replaced by E7300 and E7330

    Discontinued Date

    12/01/2012
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    Categories:
    Discontinued Products

    Description

    The NEBNext Small RNA Library Prep Set for Illumina contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the Illumina platform. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.


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    Figure 2: Agilent Bioanalyzer Trace of a Final Human Brain miRNA Library showing a 166.8 nM single peak.

    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a Illumina.

    Properties and Usage

    Materials Required but not Supplied

    3 M Sodium Acetate, pH 5.2
    100% Ethanol
    80% Ethanol
    SuperScript® II Reverse Transcriptase (Invitrogen #18064-014)
    5X First Strand Buffer (Supplied with SuperScript® II Reverse Transcriptase)
    0.1 M DTT (Supplied with SuperScript® II Reverse Transcriptase)
    Costar Spin-X centrifuge tube Filters (Cellulose Acetate Filters) (Sigma #CLS8162)
    RNase-free Disposable Pellet Pestles (Kontes #74951-1590)
    6% Novex TBE PAGE gel, 1.0 mm, 10 well (Invitrogen #EC6265BOX)
    SYBR® Gold Nucleic Acid Gel Stain (Invitrogen #S-11494)

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.
    1. Ligation of 3´ and 5´ Adaptors (E6120)
    2. Reverse Transcription (E6120)
    3. PCR Amplification (E6120)
    4. Size Selection and Gel Purification of Amplified cDNA Library (E6120)