NEBNext® Small RNA Library Prep Set for Illumina®

This product is replaced by E7300 and E7330
  • This product was discontinued on 12/01/2012
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Description

The NEBNext Small RNA Library Prep Set for Illumina contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the Illumina platform. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.


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Figure 2: Agilent Bioanalyzer Trace of a Final Human Brain miRNA Library showing a 166.8 nM single peak.

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a .

Properties and Usage

Materials Required but not Supplied

3 M Sodium Acetate, pH 5.2
100% Ethanol
80% Ethanol
SuperScript® II Reverse Transcriptase (Invitrogen #18064-014)
5X First Strand Buffer (Supplied with SuperScript® II Reverse Transcriptase)
0.1 M DTT (Supplied with SuperScript® II Reverse Transcriptase)
Costar Spin-X centrifuge tube Filters (Cellulose Acetate Filters) (Sigma #CLS8162)
RNase-free Disposable Pellet Pestles (Kontes #74951-1590)
6% Novex TBE PAGE gel, 1.0 mm, 10 well (Invitrogen #EC6265BOX)
SYBR® Gold Nucleic Acid Gel Stain (Invitrogen #S-11494)

Storage Temperature

-20°C

Protocols

  1. Ligation of 3´ and 5´ Adaptors (E6120)
  2. Reverse Transcription (E6120)
  3. PCR Amplification (E6120)

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.