NEBNext® mRNA Library Prep Master Mix Set for 454™
- This product was discontinued on May 12, 2014
The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the 454 platform.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Quick T4 DNA Ligase -20 NEBNext Sizing Buffer -20 1.2 X TE Buffer -20 NEBNext End Prep Enzyme Mix NEBNext End Repair Reaction Buffer 10 X NEBNext RNA Fragmentation Buffer -20 10 X NEBNext RNA Fragmentation Stop Solution -20 10 X Linear Acrylamide -20 10 mg/ml Random Primers -20 Murine RNase Inhibitor, 40,000 U/ml NEBNext First Strand Synthesis Reaction Buffer -20 5 X NEBNext Second Strand Synthesis Enzyme Mix -20 NEBNext Second Strand Synthesis Reaction Buffer -20 10 X Nuclease-free Water -20
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