The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.
Figure 1: Relative size distribution of eukaryotic mRNA fragments as seen using the Bioanalyzer 2100.
Poly (A)+ mRNA (40 ng) purified from the Universal Human Reference Total RNA (Stratagene) was fragmented in 1X NEBNext Magnesium RNA Fragmentation Buffer for 1–5 minutes at 94°C. Fragmentation Reaction was stopped in 1X NEBNext Fragmentation Stop Solution. Samples were diluted 1:10 in Nuclease-Free Water and analyzed in the Bioanalyzer 2100.
Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the 454 platform.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Trademarks
NEBNext® a registered trademark of New England Biolabs, Inc. LITMUS™ is a trademark of New England Biolabs, Inc.
BIOANALYZER® is a registered trademark of Agilent Technologies, Inc. AMPure® is a registered trademark of Beckman Coulter, Inc. MILLI-Q® is a registered trademark of Millipore Corporation. SuperScript® is a registered trademark of Life Technologies, Inc. RNEASY® and MINELUTE® are registered trademarks of Qiagen. 454™ and GS FLX TITANIUM™ are trademarks of Roche.
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