The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Quick T4 DNA Ligase | -20 | |
| NEBNext Sizing Buffer | 1.2 X | |
| TE Buffer | ||
| NEBNext End Prep Enzyme Mix | ||
| NEBNext End Repair Reaction Buffer | 10 X | |
| NEBNext RNA Fragmentation Buffer | 10 X | |
| NEBNext RNA Fragmentation Stop Solution | 10 X | |
| Linear Acrylamide | -20 | 10 mg/ml |
| Random Primers | ||
| NEBNext First Strand Synthesis Reaction Buffer | 5 X | |
| NEBNext Second Strand Synthesis Enzyme Mix | ||
| NEBNext Second Strand Synthesis Reaction Buffer | 10 X | |
| Nuclease-free Water | -20 |
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