The NEBNext® mRNA Library Prep Set for SOLiD™ contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.
Figure 1: Relative size distribution of eukaryotic mRNA fragments as seen using the Bioanalyzer 2100.
Figure 2: Bioanalyzer traces of mRNA Fragments with insufficient washing show a noisy baseline.
Figure 3: Final mRNA-Seq Library Bioanalyzer Trace
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the SOLiD™ 4.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration
NEBNext RNA Fragmentation Buffer 10X
NEBNext RNA Fragmentation Stop Solution 10X
Linear Acrylamide -20 10 mg/ml
Murine RNase Inhibitor
NEBNext First Strand Synthesis Reaction Buffer 5X
NEBNext Second Strand Synthesis Enzyme Mix
NEBNext Second Strand Synthesis Reaction Buffer 10X
Nuclease-free Water -20
NEBNext End Repair Reaction Buffer 10X
T4 DNA Ligase
NEBNext Quick Ligation Reaction Buffer -20 5X
LongAmp Taq 2X Master Mix 2X
NEBNext End Repair Enzyme Mix -20