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  • NEBNext® mRNA Second Strand Synthesis Module

    Description






    The NEBNext Second Strand Synthesis Module has been optimized to generate double stranded cDNA from first strand cDNA. The NEBNext Second Strand Synthesis Module is provided as a master mix to maximize efficiency and convenience in RNA sample preparation workflows. 

    The dsDNA generated by the NEBNext Second Strand Synthesis Module can be subsequently converted to blunt ended DNA fragments using the NEBNext End Repair Module. 

    The NEBNext Second Strand Synthesis Module has been validated by sequencing with the Illumina GAIIx in conjunction with M-MuLV (RNase H) Reverse Transcriptase, NEBNext First Strand Synthesis Reaction Buffer, Murine RNase Inhibitor, the NEBNext End Repair Enzyme Module, the NEBNext dA-Tailing Module, the NEBNext Quick Ligation Module, NEBNext High Fidelity 2x PCR Master Mix . 

    For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext Second Strand Synthesis Enzyme Mix
    NEBNext Second Strand Synthesis Reaction Buffer10X

    Advantages and Features

    Features

    • Efficient – Converts 10–100 ng of first strand cDNA into double stranded cDNA
    • Convenient – Reactions are provided in master mix format to reduce steps during RNA sample prep workflows
    • Automation Friendly

    Applications

    • RNA Sample Preparation
    • Second Strand Synthesis of cDNA

    Properties and Usage

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Second Strand cDNA Synthesis (E6111)