NEBNext® mRNA Library Prep Master Mix Set for Illumina®
- This product was discontinued on February 27, 2017
The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact firstname.lastname@example.org for further information.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Quick T4 DNA Ligase -20 ProtoScript® II Reverse Transcriptase -20 200,000 units/ml NEBNext End Repair Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X Klenow Fragment (3´→ 5´ exo–) -20 NEBNext dA- Tailing Reaction Buffer -20 10 X NEBNext Quick Ligation Reaction Buffer -20 5 X NEBNext RNA Fragmentation Buffer -20 10 X NEBNext RNA Fragmentation Stop Solution -20 10 X Random Primers -20 Murine RNase Inhibitor, 40,000 U/ml NEBNext First Strand Synthesis Reaction Buffer -20 5 X NEBNext Second Strand Synthesis Enzyme Mix -20 NEBNext Second Strand Synthesis Reaction Buffer -20 10 X Nuclease-free Water -20 NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
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