NEBNext® mRNA Library Prep Reagent Set for Illumina®
- This product was discontinued on February 27, 2017
The NEBNext® mRNA Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the Illumina sequencing platform.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Quick Ligation Reaction Buffer -20 2 X Phosphorylation Reaction Buffer -20 10 X Deoxynucleotide Solution Mix -20 10 mM T4 DNA Polymerase -20 DNA Polymerase I, Large (Klenow) Fragment -20 T4 Polynucleotide Kinase -20 Deoxyadenosine 5´- Triphosphate (dATP) -20 1 mM NEBuffer 2 for Klenow Fragment (3´→ 5´ exo–) -20 10 X Quick T4 DNA Ligase -20 NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE) 2 X ProtoScript® II Reverse Transcriptase -20 200,000 units/ml NEBNext RNA Fragmentation Buffer -20 10 X NEBNext RNA Fragmentation Stop Solution -20 10 X Random Primers -20 Murine RNase Inhibitor, 40,000 U/ml NEBNext First Strand Synthesis Reaction Buffer -20 5 X NEBNext Second Strand Synthesis Enzyme Mix -20 NEBNext Second Strand Synthesis Reaction Buffer -20 10 X Nuclease-free Water -20 Klenow Fragment (3´→5´ exo-) -20 NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
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